Date: November 15th
Time: 1:00 pm ET
Weill Cornell Medical College
Manager, Massively Parallel Sequencing Facility, University of Vermont Cancer Center
This webinar will provide an update on Phase 2 of the ongoing ABRF Next Generation Sequencing Study, an effort to evaluate the performance of NGS platforms and to identify optimal methods and best practices. Phase 1 of the study focused on RNA sequencing, while Phase 2 is focusing on genomic DNA samples.
In particular, Phase 2 of the ABRF NGS study aims to address three questions applicable to most technologies being used for deep sequencing of genomic DNA: 1) for a typical combination of sample preparation method and sequencing instrument (a “platform”), what levels of intra- and inter-laboratory variation should be expected; 2) how is a platform affected by DNA exposed to formalin fixation ; and 3) how is a platform affected by DNA that contains a skewed nucleotide composition? Sequencing for the study is being performed by independent academic service laboratories not affiliated with reagent or instrument vendors. The use of well-characterized, publicly available reference samples and uniform protocols within each platform will generate baseline data sets against which alternative methods, hardware upgrades, and any sequencing lab’s performance can be compared.
This webinar will outline new strategies for genome editing in mammalian cells using CRISPR/Cas9, with talks focused on point mutation repair in human cell lines and the design of knock-in animal models.
Dr. Eric Kmiec Director, Gene Editing Institute, Christiana Care Health System’s Helen F. Graham Cancer Institute & Research Center
Dr. CB Gurumurthy Director, Transgenic Core Facility, University of Nebraska Medical Center
During this webcast, Dr. Eric Kmiec will discuss a new approach to the correction of point mutations using single-stranded oligonucleotides and a partially synthetic form of CRISPR/ Cas9, a ribonucleotideprotein (RNP) complex. The experimental design, including the process of RNP assembly and the workflow, will be presented.
Dr. Kmiec will share details of a case study in which a point mutation in an integrated copy of the mutated eGFP gene in a human cell line is corrected using this approach, and a reaction pathway that is likely distinct from that of homology-directed repair. The use of short single-stranded oligonucleotides may be a strategy of choice when the desired endpoint is correction of point mutations in chromosomal genes.
Our second speaker, Dr. CB Gurumurthy, will discuss the latest trends and CRISPR tools available for animal genome editing, with a particular emphasis on strategies for increasing the homology-directed repair mechanism to enable insertion of longer sequences at the Cas9 cut sites. A few examples of designing knock-in animal models and the workflow of generating the models will be presented.
This webinar is the second on gene editing under the GenomeWeb/ABRF 2016 Webinar Series. The first webinar in the series is available on demand here.
This online seminar, part of the GenomeWeb/ABRF 2016 Webinar Series, will provide an overview of experimental and computational standards for metagenomics that have been developed as part of the Genomes in a Bottle standards consortium.
Please join Christopher Mason of Weill Cornell Medical College and Scott Tighe of the University of Vermont on June 7 at 1:00 pm EDT US for an overview of metagenomics standards that leverage a titrated mixture of known bacteria and eukaryotes. These have been sequenced across multiple next-generation sequencing platforms and characterized with ten different algorithms for taxonomic classification. The consortium members have also aggregated a set of 30 control samples for additional classification.
Dr. Mason and Dr. Tighe will report on a number of findings from the project, including the fact that sites of cross-algorithm agreement can lead to the most accurate estimate of the number of species from a new sample. They will also present an online resource for these tools, methods, and data sets; all of which are freely available. These methods and standards can help the many large-scale metagenomics projects around the world (and even some in space).
For more information and to register, please click HERE.
Under current ABRF policy, all Research Group Study Proposals are reviewed by the Executive Board prior to implementation. Over the past ten years, ABRF has expanded to include a wider array of technologies. As a result, situations may arise where the current EB members have limited backgrounds in a specific research technology area, making a comprehensive scientific review difficult. To ensure each Research Group Study Proposal receives a well-rounded review, the Executive Board would like a pool of consultants from which to solicit opinions during the approval process. All ABRF members interested in being added to the list can do so by sending an email to email@example.com with the following information:
Subject line: RG Study Proposal Consulting List
Body of the email:
– Your name
– General area of expertise (genomics, proteomics, light microscopy, flow, etc.)
– Specific area of expertise (NGS, MS, confocal microscopy, iCRISPR, etc.)
Thank you in advance for making yourself available on an RG Study Consultant list.
ABRF Executive Board
William Hendrickson, President
Paula Turpen, Treasurer
Frances Weis-Garcia, President-Elect
Christopher Colangelo, Treasurer-Elect